Shimadzu GC-2025 — Gas Chromatograph with FID

The Shimadzu GC-2025 is a gas chromatograph equipped with a Flame Ionization Detector (FID), used for the separation and quantification of volatile and semi-volatile organic compounds.

Location: ⚠️ To be completed — building, room number Responsible: ⚠️ To be completed


Instrument Specifications

ParameterValue
Injection SystemSplit/Splitless (SPL)
DetectorFlame Ionization Detector (FID)
Minimum Detection2 pgC/s
Dynamic Range10⁷
Jet MaterialFused quartz
Time Constant4 ms to 2 s (selectable)

📋 From manual — 221-79103D_GC-2025_IM_EN.pdf p.3–4, p.117

Temperature Ranges

ComponentRangeIncrementOverheat Protection
Column Oven0.0–450.0 °C0.01 °CN/A
Injection PortUp to 400 °C0.1 °CUp to 420 °C
FID DetectorUp to 400 °C0.1 °CUp to 420 °C

Temperature programming supports up to 20 ramps, rates from −250 to +250 °C/min in 0.01 °C/min increments.

📋 From manual — 221-79103D_GC-2025_IM_EN.pdf p.3–4

Carrier Gas Options

GasRecommended Purity
Helium (He)≥ 99.999%
Nitrogen (N₂)≥ 99.999%
Hydrogen (H₂)≥ 99.999%
Argon (Ar)

📋 From manual — 221-79103D_GC-2025_IM_EN.pdf p.114

Column and Stationary Phase

⚠️ To be completed — column type installed, dimensions, stationary phase


Injection Modes

The GC-2025 supports three injection modes via the SPL unit — see Split_vs_Splitless_Injection for detailed theory and selection guidance:

  • Split — for high-concentration samples
  • Splitless — for trace-level analysis
  • Direct — used only for SPL leak checks

📋 From manual — 221-79103D_GC-2025_IM_EN.pdf p.3


How It Works

The GC-2025 uses two fundamental principles:

  • Gas chromatographic separation — compounds partition between a carrier gas and a stationary phase inside a column, eluting at characteristic retention times → GC_Separation_Theory
  • Flame ionization detection — eluting compounds burn in a hydrogen/air flame, generating ions measured as electrical current → Flame_Ionization_Detection

Applications at UFAZ

⚠️ To be completed — add specific analyses, common sample types, student projects


PageDescription
Quick_Start_GuideStartup → run one sample → shutdown
Method_DevelopmentCreating and saving method files
Quantification_MethodsCalibration and quantitation setup
Single_Run_AnalysisRunning a single sample
Batch_ProcessingAutomated sequence analysis
Data_AnalysisPostrun chromatogram review, peak integration, peak identification
Calibration_ReviewEvaluating calibration curves and requantifying unknowns
ReportingGenerating and printing reports
Data_ReprocessingApplying a new method to old data
Sample_PreparationPreparation techniques
MaintenanceMaintenance schedules
TroubleshootingProblem solving
ReferencesManuals and technical documents

Theory

PageDescription
GC_Separation_TheoryChromatographic principles, key parameters
Flame_Ionization_DetectionFID mechanism, strengths, blind spots
Split_vs_Splitless_InjectionInjection mode selection